Research Paper Volume 14, Issue 12 pp 5250—5270
TRPM7 via calcineurin/NFAT pathway mediates metastasis and chemotherapeutic resistance in head and neck squamous cell carcinoma
Figure 4. Combination of anti-TRPM7 shRNA and cisplatin enhances cytotoxicity in ALDH+ HNSCC cells. (A) Box-plot diagrams of the relationship of TRPM7 expression with cisplatin sensitivity/resistance according to the GEO dataset GSE117872. (B) Significant inhibitory effect of the combination of TRPM7 shRNA and cisplatin on tumorspheres. (C) Wound healing analysis by using cells transfected with TRPM7 shRNA and cisplatin in SAS and FaDu cells. (D) Association of TRPM7 with possible regulatory pathways in Western blot assays. (E) Apoptosis analysis of SAS and FaDu cells with or without TRPM7 shRNA.*p < 0.05, **p < 0.01. Scale bar: 100 μm.