Research Paper Volume 14, Issue 13 pp 5406—5416

Figure 4. MSH2 was the downstream target of miR-370-3p in cervical cancer. (A) On the basis of the TargetScan data resource, MSH2 mRNA was the putative downstream target of miR-370-3p. (B) The contents of MSH2 mRNA in ten paired cervical cancer samples and vicinal non-malignant tissues. (C) The relative contents of MSH2 mRNA in normal HcerEpic cell line and cervical cancer cell lines. (D, E) Dual luciferase reporting experiments revealed that the relative amount of fluorescence value was reduced after transfected with miR-370-3p in SiHa, as well as HT-3 cervical cancer cell lines. (F, G) Abundance of circEPSTI1, MSH2 mRNA and miR-370-3p on the AGO2 target protein, assessed via RIP assays. (H, I) Enrichment of AGO2 protein to circEPSTI1 was diminished whilst MSH2 mRNA was increased after silencing of circEPSTI1 in SiHa along with HT-3 cervical cancer cell lines.