Research Paper Volume 14, Issue 16 pp 6507—6519

Figure 3. miR-141 represses EGFR to suppress expression of β-catenin. (A, B) RT-qPCR and Western blot assays were used to detect the expression of EGFR and β-catenin in lung tissues of mice and PMVECs with I/R injury. (C) the predicted binding sites between miR-141 and EGFR based on TargetScan database. (D) the dual luciferase reporter gene assay proved that validation of the binding relationship between miR-141 and EGFR. (E) RT-qPCR and Western blot assays were used to detect the expression of EGFR and β-catenin in response to silencing miR-141 in H/R cell model. (F) RT-qPCR assay was used to detect the silencing efficiency of si-EGFR-1/-2/-3 in H/R-exposed mouse PMVECs. (G) The expression of miR-141, EGFR and β-catenin in PMVECs by RT-qPCR and Western blot analysis. (H) Pearson correlation analysis of the correlation between miR-141 and EGFR, between miR-141 and β-catenin, as well as between EGFR and β-catenin. * p < 0.05 was considered statistically significant. The experiment was repeated three times independently. Results were expressed as the mean ± SD.