Research Paper Volume 14, Issue 14 pp 5669—5680

Figure 3. Effect of STAT1 inhibitor pretreatment on KLF4 overexpression. (A, B) The proportion of M1 cells was detected by flow cytometry (n=3): STAT1 inhibitor suppressed the M1 polarization of macrophages and decreased the cell proportion.**P<0.01 between groups. The proportion of M1 cells in L/I+KLF4+Cerulomvcin was lower than that in group L/I+KLF4. (C) IF staining of CD86 expression (n=3): STAT1 inhibitor suppressed CD86 expression and markedly decreased the fluorescence intensity. (D) RIP assay indicated the binding relation between KLF4 and STAT1. (E) Expression of M1 cell marker proteins (n=3, TNF-α, IL-6, IL-1β). After STAT1 inhibitor pretreatment, the expression of inflammatory factors TNF-α, IL-6 and IL-1β was down-regulated. **P<0.01 between groups. L/I+KLF4+Cerulomvcin was lower than that in group L/I+KLF4. (F) Expression of mRNA (n=3, TNF-α, IL-6, IL-1β). After STAT1 inhibitor pretreatment, the expression of mRNA was down-regulated. **P<0.01 between groups. L/I+KLF4+Cerulomvcin was lower than that in group L/I+KLF4. (G, H) Expression of JAK1-STAT1 signal proteins (n=3). After STAT1 inhibitor pretreatment, the expression of JAK1 and STAT1 proteins decreased, and their phosphorylation levels were down-regulated. **P<0.01 between groups.