Research Paper Volume 14, Issue 14 pp 5669—5680

Figure 2. Effect of KLF4 silencing on M1 polarization of macrophages. (A, B) The proportion of M1 cells was detected by flow cytometry (n=3): KLF4 silencing (siRNA-KLF4) decreased the F4/80+CD86+ cell proportion, The proportion of cells in siRNA-KLF4 was lower than that in L/I. **P<0.01 between groups. (C) IF staining of CD86. KLF4 silencing suppressed CD86 expression, and decreased the fluorescence intensity. (D) Expression of M1 cell marker proteins (n=3, TNF-α, IL-6, IL-1β). KLF4 silencing (siRNA-KLF4) suppressed the expression of inflammatory factors TNF-α, IL-6 and IL-1β. Compared with L/I, the expression of cytokines decreased significantly**P<0.01 between groups. (E) Expression of mRNA (n=3, TNF-α, IL-6, IL-1β). KLF4 silencing (siRNA-KLF4) suppressed the expression of mRNA. Compared with L/I, the expression of cytokines decreased significantly**P<0.01 between groups. (F, G) Expression of JAK1-STAT1 signal proteins (n=3). KLF4 silencing suppressed the expression of JAK1 and STAT1 proteins, decreased the phosphorylation levels. **P<0.01, relative protein expression between groups. There was no difference between Control and BsiRNA-KLF4, but siRNA-KLF4 was significantly lower than L/I.