Research Paper Volume 14, Issue 11 pp 4914—4926

Figure 6. SOX9 is the downstream target gene of miR-485-3p. (A) The expression level of SOX9 in NPC and para-tumor tissues was analyzed based on the TCGA database. (B) The person analysis shows the miR-485-3p and circSOX9 expression correlation in NPC tissues. (C, D) qRT-PCR and Western blot analysis of SOX9 expression in HONE1 and CNE2 cells after miR-485-3p inhibition or overexpression. (E) The predicted binding site of miR-485-3p and SOX9. (F) Dual-luciferase reporter gene assay was used to prove the binding of SOX9 and miR-485-3p. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.