Research Paper Volume 14, Issue 11 pp 4914—4926

Figure 3. CircSOX9 promotes the proliferation and migration of HONE1 and CNE2 cells. (A) qRT-PCR was used to measure the effect of circSOX9 knockdown in HONE1 or CNE2 cells. (B, C) CCK8 detects cell proliferation ability of HONE1 and CNE2 after knocking down circSOX9. (D) Clone formation experiment detects the proliferation ability of HONE1 and CNE2 after knocking down circSOX9. (E) Transwell assay detects the invasion and migration ability of HONE1 after knocking down circSOX9. (F) After knocking down circSOX9 in HONE1 or CNE2 cells, qRT-PCR and western blot assays were used to detect the expression of EMT-related markers Vimentin, N-cadherin, and Twist1. (G) Immunofluorescence analysis of Vimentin and E-cadherin expression in NPC cells, scale bar = 50 μm. ^**P < 0.01, ^***P < 0.001.