Research Paper Volume 14, Issue 11 pp 4858—4873
A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer
Figure 3. ALG10 knockdown attenuates the tumor-initiating ability of CRC cells. (A) Tumor images derived from HCT-116 cells with or without ALG10 knockdown at different cell concentrations. (B) Tumor formation ratio was calculated for HCT-116 cells with or without ALG10 knockdown at different cell concentrations. (C) The 1/(stem cell frequency) was determined for HCT-116 cells with or without ALG10 knockdown using an ELDA: Extreme Limiting Dilution Analysis (http://bioinf.wehi.edu.au/software/elda/). (D) The difference in stem cell frequencies between the two groups described in (C) was measured using an ELDA: Extreme Limiting Dilution Analysis (http://bioinf.wehi.edu.au/software/elda/). (E) Tumor images derived from HT-29 cells with or without ALG10 knockdown at different cell concentrations. (F) Tumor formation ratio was calculated for HT-29 cells with or without ALG10 knockdown at different cell concentrations. (G) The 1/(stem cell frequency) was determined for HT-29 cells with or without ALG10 knockdown using an ELDA: Extreme Limiting Dilution Analysis (http://bioinf.wehi.edu.au/software/elda/). (H) The difference in stem cell frequencies between the two groups described in (G) was measured using an ELDA: Extreme Limiting Dilution Analysis (http://bioinf.wehi.edu.au/software/elda/).