Research Paper Volume 14, Issue 11 pp 4755—4768

Figure 8. Tumorigenesis experiments in nude mice in vivo proved the effects of LINC00094 and miR-19a-3p on breast cancer. (A) 5×10⁶ BT-474 cells with stable expression of miR-19a-3p were injected into the breast fat pad of 4-week-old female BALB/c nude mice, and tumors were collected on the 15th day of tumor formation. (B) The tumor size of nude mice was measured every 3 days. (C) 5×10⁶ BT-474 cells statically expressed by LINC00094 were injected into the breast fat pad of 4-week-old female BALB/c nude mice, and tumors were collected on the 15th day of tumor formation. (D) Tumor size of nude mice was measured every 3 days. 3-5 nude mice of LINC00094 stable expression group and control group were respectively. There was statistical difference between experimental group and control group. ***P<0.001. (E) On the 15th day, the tumor of nude mice in each group was taken and weighed. There were statistical differences between the experimental group and the control group. *P<0.05, **P<0.01. (F) Tumor tissues of nude mice were stained with HE. (G) Western Blot was used to detect CYP19A1 protein expression level of the first pair to the third pair, with GAPDH as internal reference, and the experiment was repeated for 3 times. N is the control group. T is the experimental group. ***P<0.001, ****P<0.0001. (H) Western Blot was used to detect CYP19A1 protein expression level of the 4th to 6th pairs, with GAPDH as internal reference, and the experiment was repeated for 3 times. N is the control group. T is the experimental group. ***P<0.001, ****P<0.0001.