Research Paper Volume 14, Issue 10 pp 4486—4499

Figure 4. miR-128-3p promotes the viability and osteoclast differentiation of RAW 264.7 cells. (A) qRT-PCR for relative expression of miR-128-3p in bone tissues (three non-osteoporotic and three osteoporotic). (B) RAW 264.7 cells were transfected with miR-128-3p mimic or miR-128-3p inhibitor, respectively. The expression levels of miR-128-3p were measured by qRT-PCR. (C–E) The mRNA levels of c-Fos, NFATc1, and Ctsk were analyzed by qRT-PCR, respectively. (F) The influence on cell proliferation ability of miR-128-3p mimic and miR-128-3p inhibitor was assessed by CCK-8 assay. (G and H) The protein levels of c-Fos, NFATc1, and Ctsk were analyzed by Western blotting, respectively. (I) Transwell assay detecting the migration ability of RAW 264.7 cells (Scale bar: 100 μm). (J) Representative images of TRAP-positive osteoclasts formation (Scale bar: 200 μm). ^*P < 0.05, ^**P < 0.01; ^***P < 0.001. Abbreviation: ns: not significant.