Figure 2.KCNQ1OT1 inhibits the osteoclast differentiation of RAW 264.7 cells. (A–E) RAW 264.7 cells transfected with pcDNA-KCNQ1OT1, si-KCNQ1OT1, or corresponding control vectors and treated with RANKL for 5 days, the mRNA and protein levels of the osteoclastogenesis markers c-Fos, NFATc1, and Ctsk were measured by qRT-PCR and Western blotting. (F) A Tartrate-resistant acid phosphatase (TRAP) staining kit was used for osteoclast staining, and TRAP-positive multinucleated cells were counted (Scale bar: 200 μm). *P < 0.05, **P < 0.01; ***P < 0.001, ns: not significant.
