Research Paper Volume 14, Issue 9 pp 4137—4157
LncRNA CRART16/miR-122-5p/FOS axis promotes angiogenesis of gastric cancer by upregulating VEGFD expression
Figure 8. CRART16 functions as a miR-122-5p sponge, and miR-122-5p targets FOS mRNA. (A) The predicted binding site between CRART16 and miR-122-5p is illustrated. The luciferase activities were determined after co-transfection with either miR-122-5p mimics or negative control mimics and pGL3 encoding wild-type or mutated (Mut) CRART16 (**P < 0.01). (B) The putative binding site between miR-122-5p and the 3’-UTR of FOS mRNA is illustrated. The luciferase activities were determined after co-transfection with miR-122-5p mimics or negative control mimics and pGL3-FOS carrying the wild-type or mutated (Mut) 3’UTR (**P < 0.01). All data are presented as mean ± SD from three separate experiments.