Research Paper Volume 14, Issue 9 pp 4137—4157
LncRNA CRART16/miR-122-5p/FOS axis promotes angiogenesis of gastric cancer by upregulating VEGFD expression
Figure 6. CRART16 overexpression increases c-Fos and VEGFD expression by targeting miR-122-5p. (A) The relative levels of miR-122-5p and mRNAs encoding FOS and VEGFD in different gastric cancer cell lines were analyzed by qRT-PCR (*P < 0.05). (B) The relative levels of c-Fos and VEGFD in different gastric cancer cell lines were determined by western blotting. GAPDH was used as a loading control (*P < 0.05). (C) The expression levels of VEGFD in conditioned media from different types of gastric cancer cells were determined by ELISA (**P < 0.01). All data are presented as mean ± SD from three separate experiments.