Figure 3. PRDX5 knockdown enhanced the ability of PAM to inhibit the malignant behavior of A549 lung cancer cells. (A) The day after cancer cells had attached to the bottom of plates and the medium was removed following PAM treatment. Upper panel: the colony-forming ability in A549 cells exposed to PAM for 7 days as detected via colony formation assays. (B) Quantification of colony formation data. (C) Images of the wound healing assay. (D) Quantification of wound healing area. The data are presented as the mean ± standard deviation of three independent experiments. Significant differences are indicated at *p < 0.05; **p < 0.01; ***p < 0.001 vs. the control. Con, control.