Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 4. PIAS1 interacts with UBE2I. (A) The online biological database was used to predict the interaction relationship UBE2I between and PIAS1 (https://inbio-discover.intomics.com/map.html). (B) The binding relationship of UBE2I and PIAS1 was validated by co-immunoprecipitation assay. (C) Co-immunoprecipitation assay was used to validate the binding relationship of NRIP1, UBE2I and PIAS1. (D) The pcDNA-UBE2I or si-UBE2I was transfected into cells, and the protein expression of UBE2I and PIAS1was analyzed by Western botting. (E) The cells were transfected with pcDNA-NRIP1 alone or together with si-UBE2I, and the protein expression of PIAS1was analyzed by Western botting. GAPDH was used as an internal reference. N = 6, *P < 0.01.