Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 2. UBE2I interacts with NRIP1. (A) The online biological database was used to predict the interaction relationship NRIP1 between and UBE2I (https://inbio-discover.intomics.com/map.html). (B) The pcDNA-NRIP1 or si-NRIP1 was transfected into TC-1 cells, respectively, and the protein expression of UBE2I was detected by Western blotting. (C) The binding relationship of NRIP1 and UBE2I was validated by co-immunoprecipitation assay. GAPDH was used as an internal reference. N = 6, *P < 0.01.