Research Paper Volume 14, Issue 7 pp 3216—3232

Figure 7. Trehalose inhibits ferroptosis by activating the NRF2/HO-1 signaling pathway. (A–C) Western blot to detect the expression of NRF2 and HO-1 protein and protein quantitative analysis; (D, E) Immunofluorescence evaluation of NRF2 expression and fluorescence quantification analysis; (F, G) Western blot to detect the expression of NRF2, HO-1, Acsl4 and Gpx4 protein and quantitative protein analysis; (H, I) Immunofluorescence to evaluate the expression of NRF2 and quantitative fluorescence analysis; (J–L) Fe²⁺, MDA and GSH measurement; (M) CCK8 method is used to assess cell viability; (N) PI staining of neuronal cells; (O) Quantification of PI positive cells. ^*p < 0.05 compared with the Control group or Erastin+Trehalose group, ^#p < 0.05 compared with the Erastin group.