Research Paper Volume 15, Issue 16 pp 8518—8527
miR-153-3p suppresses the differentiation and proliferation of neural stem cells via targeting GPR55
Figure 6. miR-153-3p inhibits NSC differentiation and proliferation by targeting GPR55 expression. (A) Cell proliferation was measured using CCK-8 analysis. (B) Nestin expression was determined by qRT-PCR. (C) The expression of Tuj1 was detected by qRT-PCR. (D) The expression of GFAP was measured by qRT-PCR. (E) The GPR55 antagonist ML-193 inhibited cell growth compared with the vehicle group in miR-153-3p-treated NSCs. (F) Nestin expression was determined by qRT-PCR analysis. (G) The expression of Tuj1 was detected by qRT-PCR. (H) The expression of GFAP was measured by qRT-PCR. *p<0.05 and **p<0.01. Error bars represent the s.d. of relative experiment from n=3 replicates.