Figure 4. miR-153-3p suppresses NSC differentiation and proliferation. (A) Ectopic expression of miR-153-3p inhibited NSC proliferation. (B) Overexpression of miR-153-3p decreased nestin expression. (C) The expression of Tuj1 was detected by qRT-PCR. (D) The expression of GFAP was measured by qRT-PCR. (E) The expression of miR-153-3p was measured by qRT-PCR. (F) The suppression of miR-153-3p increased NSC growth. (G) Nestin expression was measured by qRT-PCR. (H) The expression of Tuj1 was detected by qRT-PCR. (I) The expression of GFAP was measured by qRT-PCR. *p<0.05, **p<0.01 and ***p<0.001. Error bars represent the s.d. of relative experiment from n=3 replicates.