Research Paper Volume 15, Issue 16 pp 8518—8527
miR-153-3p suppresses the differentiation and proliferation of neural stem cells via targeting GPR55
Figure 3. GPR55 is a direct gene target of miR-153-3p. (A) The expression of GPR55 was determined by qRT-PCR. (B) One potential target site was found between miR-153-3p and GPR55. These sequences were conserved between different species. (C) The expression of GPR55 was determined by qRT-PCR. (D) Luciferase reporter analysis noted that elevated expression of miR-153-3p significantly inhibited the luciferase value of the WT reporter plasmid but did not change the luciferase value of the mut reporter plasmid. **p<0.01 and ***p<0.001. Error bars represent the s.d. of relative experiment from n=3 replicates.