Figure 7.Levobupivacaine attenuates cell proliferation and CSCs properties of osteosarcoma cells by regulating KAT5/MAFB. (A–E) MG63 and U2OS cells were treated with levobupivacaine, or co-treated with levobupivacaine and KAT5 or MAFB overexpression vectors. (A,B) Cell proliferation was detected by Edu assays. (C, D) Cell apoptosis was analyzed by Annexin V/PI apoptosis detection kit. (E) The stemness was measured by sphere formation assays. mean ± SD, ** P < 0.01.
