Research Paper Volume 14, Issue 6 pp 2748—2757

Figure 5. IP protected HUVECs against OGD/R via downregulating miR-92a, miR-328 and miR-494. To construct a model of OGD/R in vitro, HUVECs were cultured in glucose-free and serum-free DMEM at 95% N₂ and 5% CO₂ for 4 h. Then, HUVECs were cultured in DMEM containing 10% serum at 95% N₂ and 5% CO₂ for 18 h. (A) CCK-8 staining assay was used to detect the viability of HUVECs. (B) EdU staining assay was used to detect the proliferation of HUVECs. (C–E) RT-qPCR was performed to detect the expression of miR-92a, miR-328 and miR-494 in HUVECs. **P<0.01 compared with the sham group; ^##P<0.01 compared with the IR group.