Figure 3. IP protected myocardial tissues against IR injury. (A) H&E staining assay was conducted to observe the morphology of nonculprit myocardial tissues. (B) TUNEL and DAPI staining assay was performed to detect the apoptosis of nonculprit myocardial tissues. (C–E) RT-qPCR assay was used to detect the expression of AT1, Cx43 and β-tubulin. **P<0.01 compared with the sham group; ##P<0.01 compared with the IR group.