Priority Research Paper Volume 14, Issue 6 pp 2442—2461
Regulation of mTOR complexes in long-lived growth hormone receptor knockout and Snell dwarf mice
Figure 3. Decreased DEPTOR and PRAS40 in immunoprecipitated mTOR complexes. (A) Representative immunoblots of the mTORC1 components from samples immunoprecipitated with anti-RAPTOR antibody. (B) Representative immunoblots of the mTORC2 components immunoprecipitated with anti-RICTOR antibody. (C) Representative immunoblots of the input samples. (D) Quantification of the protein levels in RAPTOR-IP samples, as in (A). (E) Quantification of the protein levels in RICTOR-IP samples, as in (B), for N = 6 male mice, with mean and SEM. *t-test p < 0.05.