Research Paper

Figure 6. CIRCRBM33 regulates the tumor growth in vivo. (A–C) The SCID nude mice were randomly divided into two groups, then MCF7 cells (1×10⁶/100μL/mouse) transfected with si-control (si-NC) si-circRBM33, or co-transfected with si-circRBM33 and CBX7 overexpressing plasmid were hypodermically injected in the nude mice. The width and length of tumor, and the body weight of mice were measured at indicated time. Tumor volume was calculated by the formula: width (mm)² × length (mm)/2. The mice were anesthetized to death when tumor size reached 1000 mm³, and the tumors were collected. (A) The tumor volume was shown. (B) The tumor weight was shown. (C) The expression of PITPNA-AS1, miR-17a-5p, CBX7 was determined. N=5. Data are presented as mean ± SEM. Statistic significant differences were indicated: ** P < 0.01, ## P < 0.01.