Research Paper
Circular RNA RBM33 promotes breast cancer progression by targeting miR-17-5p/CBX7 signal pathway
Figure 3. CircRBM33 acts as a sponge for miR-17a-5p. (A, B) Subcellular fractionation assay was performed to identify the location of circRBM33; (C) The predicted target of circRBM33; (D) The underlying target level of circRBM33, miR-17-5p, miR-33a-5p, miR-20b-5p; (E) The binding sites between circRBM33and miR-17a-5p; Luciferase activity of miR-17a-5p mimic with PITPNA-AS1-WT or PITPNA-AS1-MUT; (F) AGO2 RIP assay; (G) Pull-down assay. (H) MiR-17a-5p expression in breast cancer cell. Data are presented as mean ± SEM. Statistic significant differences were indicated: ns, no significance, * P < 0.05, ** P < 0.01.