Research Paper
Circular RNA RBM33 promotes breast cancer progression by targeting miR-17-5p/CBX7 signal pathway
Figure 2. Wnt signal activation prevents si-circRBM33 effect on breast cancer cells. (A–D) MCF-7 and MDA-MB-231 cells were treated with circRBM33 siRNA or co-treated with circRBM33 siRNA and Wnt agonist 1 (10 μM). (A) The cell cycle was determined by flow cytometry analysis. (B) The cell proliferation was measured by clone formation assay. (C) The cell invasion was detected by Transwell assay. (D) The cell apoptosis was assessed by TUNEL assay. (E) The expression of Bax, cleaved caspase-3, caspase-3, and Bcl-2 was tested by Western blot analysis. Data are presented as mean ± SEM. Statistic significant differences were indicated: * P < 0.05 vs. si-NC, #P < 0.05 vs. si-circRBM33.