Research Paper Volume 14, Issue 6 pp 2574—2589
Progesterone attenuates neurological deficits and exerts a protective effect on damaged axons via the PI3K/AKT/mTOR-dependent pathway in a mouse model of intracerebral hemorrhage
Figure 2. Effect of progesterone on neuron death and cell apoptosis. (A) Representative immunofluorescence staining images of NeuN (red) in perihematomal region. Nuclei were counterstained with DAPI (blue). Bar=50μm. (B) Quantitative analyses of NeuN relative fluorescent intensity in perihematomal region in each group. (C) Representative micrographs of TUNEL staining in perihematomal region in each group. Bar=50μm. (D) Quantitative analysis shows that TUNEL positive cells in perihematomal region. (E) Representative Western bands showing the protein expression of Bcl-2 and Bax in perihematomal region. (F, G) Quantitative analysis of Western blots shows that the expression of Bcl-2 and Bax changes in each group. (H) Representative Nissl-stained images in perihematomal region in each group. Bar=50μm. n = 6 animals per group. Data are expressed as the mean ± SEM; **P < 0.01 vs. sham; #P < 0.05 vs. ICH group; ##P < 0.01 vs. ICH group. PROG: progesterone.