Priority Research Paper Volume 14, Issue 5 pp 2047—2061

Figure 6. Primary MEF Zmpste24 KO cells exhibit a blockade of the cell cycle in the basal state. (A) Immunoblot analysis of Lamin A (in Primary MEF Zmpste24 WT cells), prelamin A (in Primary MEF Zmpste24 KO cells), p27 and LC3B II–I ratio using β actin as loading control, in the cell extracts with FBS reduction (n = 3). (B) The plot indicates the quantification data of Lamin A or prelamin A/β-actin ratio, p27/β-actin ratio and LC3B II/LC3B I ratio of Primary MEF Zmpste24 WT and KO in both in growth state and in nutrient deprivation. Data represent the mean ± standard error of the mean (SEM). Differences were determined by Dunnett's multiple comparisons test.