Priority Research Paper Volume 14, Issue 5 pp 2047—2061
Cell immortalization facilitates prelamin A clearance by increasing both cell proliferation and autophagic flux
Figure 2. Immortalized MEF Zmpste24 KO cells present an increase in ER-stress. (A) Immunoblot analysis of BIP, P-PERK, PERK, eiF2α and P- eiF2α using both, β actin and Tubulin as loading control, in the cell extracts in basal state (n = 5). The plot indicates the quantification data of eiF2α/β-actin ratio, P-eiF2α 62/eiF2α ratio, BIP/Tubulin ratio and P-PERK/PERK ratio in the basal state. Data represent the mean ± standard error of the mean (SEM). Differences were determined by unpaired Student t-test analysis. *p < 0,05; **p < 0,01; ***p < 0,005 (n = 5). (B) Electron microscopy of ER of MEF Zmpste24 WT and KO cells in basal state. The plot indicates the quantification data of ER dilation using image J. *p < 0,05.