Research Paper Volume 14, Issue 5 pp 2335—2347
Decrease in ovarian reserve through the inhibition of SIRT1-mediated oxidative phosphorylation
Figure 5. Res improves ovarian function by activating SIRT1. (A) No changes in body weight. (B) Ex-527 and 3-NPA prolonged the mean length of the diestrus phase and decreased the mean length of the estrous phase, while Res had the opposite effect. (C) Ex-527 and 3-NPA decreased the ovarian index and Res increased the ovarian index. (D) Ex-527 and 3-NPA increased serum FSH levels, and Res decreased these levels. (E) Ex-527 and 3-NPA decreased serum AMH levels and Res increased these levels. (F) Ex-527 and 3-NPA decreased the number of primordial follicles. (G) Ex-527 and 3-NPA decreased SIRT1 activity, while Res increased it. (H, I) Ex-527 and 3-NPA could decrease SOD (H) and GSH-Px (I) activity, while Res could increase SOD (H) and GSH-Px (I) activity. (J) Ex-527 and 3-NPA increased MDA activity, while Res decreased this activity (N=8 in all assays; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).