Research Paper Volume 14, Issue 5 pp 2335—2347

Figure 2. 40 mg/kg 3-NPA significantly induced manifestations similar to those of POI. (A) 40 mg/kg 3-NPA could prolong the mean length of the diestrus phase and decrease the mean length of the estrous phase after three weeks of treatment. (B) 40 mg/kg 3-NPA could decrease the number of primordial follicles and increase that of atretic follicles. (C–F) 40mg/kg 3-NPA decreased the ovarian index (C), increased serum FSH levels (D), decreased serum E₂ levels (E), decreased serum AMH levels (F), and decreased mouse fertility (G). (H) Although the 40 mg/kg 3-NPA group had fewer pups, the difference was not significant. (I, J) The offspring of the mice treated with 40 mg/kg 3-NPA showed lower weight than the control group (N=8 in all assays; *P<0.05, **P<0.01).