Research Paper Volume 14, Issue 5 pp 2131—2147

Figure 2. Isolation and enrichment strategy of primary NK cells from human PBMC. (A) Experimental design of the NK cell enrichment strategy. PBMCs were collected from multiple donors (ages 20-42 years old), and NK cells were isolated and enriched. (B) Flow cytometry analysis of CD56 and CD16 expression in NK cells before and after enrichment for a representative donor. (B-i) Before enrichment, 1.64% of NK cells (0.18% of PBMCs) were CD56^Bright CD16^- and 46.34% of NK cells (2.8% of PBMCs) were CD56^Dim CD16⁺ NK cells. (B-ii) After enrichment, 2.29% of NK cells (0.47% of PBMCs) were CD56^Bright CD16^- and 66.18% of NK cells (12.6% of PBMCs) were CD56^Dim CD16⁺ NK cells. (C-i) Average percentage of CD56^Dim CD16⁺ NK cell population in PBMCs from five donors. (C-ii) Average percentage of CD56^Bright CD16^- NK cell population in PBMCs from five donors. Donor sex and age are indicated in the figure. Statistical analysis performed using paired t test. *p < 0.05, **p < 0.01, and ***p < 0.001.