Research Paper Volume 14, Issue 5 pp 2081—2100

Figure 1. In vitro cytotoxicity induced by FD-895 and pladienolide B in different cancer cell lines, and normal human primary PBMCs. Cancer Cells were exposed to FD-895 (100 nM to 2 uM), pladienolide B (100 nM to 2 uM), etoposide (1 μM to 30 μM), and cisplatin (1 μM to 30 μM) for 48 h. Apoptosis were measured in MCF-7 (A), MDA-MB-468 (B), HCT-116 (C) and HeLa (D) cells using MTS assay. The absorbance of the control (cells without treatment) was subtracted from the treated cells of each cell line. (E) Normal PBMC cells were exposed to FD-895, and pladienolide B. Cells were stained with propidium iodide and DiOC₆ to differentiate dead and viable cells by using flow cytometer. Data presented in form of % specific induced apoptosis (% SIA). To assess the compound specific induced apoptosis vs. background spontaneous cell death from in vitro culture conditions, we calculated the percentage of SIA using the following formula: % SIA = [(compound induced apoptosis – media only spontaneous apoptosis)/(100- media only spontaneous apoptosis)] × 100. The data shows the results of samples analyzed in duplicate with the mean and its respective SD. (F) Structures of pladienolide-B and FD-895.