Research Paper Volume 14, Issue 4 pp 1782—1796

Figure 8. Overexpression of ROCK1 altered changes in cell viability, apoptosis, colony formation ability, migration and invasion ability, caused by miR-154-3p mimics. (A) qRT-PCR assay for ROCK1 mRNA expression in HepG2 cells, transfected with miR-154-3p mimics and with or without ROCK1 ov. (B) Cell viability assay was carried out in HepG2 cells transfected with miR-154-3p mimics and with or without ROCK1 ov. (C) Colony formation ability of HepG2 cells that were transfected with miR-154-3p mimics and with or without ROCK1 ov. Quantitative analysis was performed of the colonies were formed (right panel). (D) Apoptosis assays of HepG2 cells transfected with either miR-154-3p mimics and with or without ROCK1 ov, and quantitative analysis was performed of the apoptosis cells (right panel). (E, F) The migration and invasion ability of HepG2 cells transfected with miR-154-3p mimics and with or without ROCK1 ov were determined using the transwell migration assay (E) and transwell invasion assay (F) (left panel). Quantitative analysis of the migratory or invasive cells in transwell assays was also performed (right panel). (G) Migratory ability of HepG2 cells transfected with miR-154-3p mimics and with or without ROCK1 ov at indicated time points, as evaluated by wound healing assay (upper panel). Quantitative analysis of the gap of wound healing assay (lower panel) was performed at 48 hours. *p < 0.05.