Research Paper Volume 14, Issue 4 pp 1743—1766
THRSP identified as a potential hepatocellular carcinoma marker by integrated bioinformatics analysis and experimental validation
Figure 8. The effect of THRSP expression on cell apoptosis and channel regulation in HCC. (A, B) The apoptosis of HCCLM3 cells detected by flow cytometry. (C–F) The expression of apoptosis-related molecules (bax, bcl-2 and caspase 3) and MAPK/NF-κB pathway-related molecules (erk, p-erk, p38, p-p38, p65 and p-p65) examined by qRT-PCR or Western blotting assays. (G) Each of the protein bands. The dividing lines (dashed lines) indicated that the grouping of images were from different parts of the same gel. (H–J) Invasion and migration rates were analyzed when treated with AG-126, SB203580, SP600125 and BAY-11-7082 inhibitors.