Priority Research Paper Volume 14, Issue 3 pp 1068—1086

Figure 2. Senostatic and senolytic action of drugs. NIH-3T3 cells were treated with a single dose of radiation (0 Gy or 17.5 Gy). (A–D) Six hours after irradiation, cells were treated with INK-128 or Rapamycin. The percentage of cellular senescence, as determined by SA-β-Galactosidase activity, and the relative cell count were assessed five days after drug treatment. (E, F) NIH-3T3 cells treated with a single dose of radiation (0 Gy or 17.5 Gy). Three days after irradiation, cells were treated with ABT-737. Two days after drug was applied the percentage of cellular senescence, as determined by SA-β-Galactosidase activity, and the relative cell number were assessed. Relative cell counts for each drug is normalized to vehicle from the respective group (0 Gy or 17.5 Gy). (G) SA-β-Galactosidase activity of NIH-3T3 cells 5 days after irradiation and treated with vehicle, INK-128 (100nM), Rapamycin (100 nM) or ABT-737 (1μM) (Scale bar: 100 μm). ^§: indicates p < 0.05 compared to 0 Gy, Vehicle. ^*: indicates p < 0.05 compared to 17.5Gy, Vehicle.