Research Paper Volume 14, Issue 3 pp 1321—1335
CTRP3 alleviates cardiac ischemia/reperfusion injury via LAMP1/JIP2/JNK signaling pathway
Figure 6. CTRP3 and LAMP1 overexpression accelerated activation of JIP2/JNK signaling pathway. (A) The relationship between CTRP3 and JIP2 was detected with Co-IP. (B, C) The relationships among LAMP1, NUMB and JIP2 were detected by using Co-IP. (D–F) Relative protein expression of JIP2 and p-JNK was detected with Western blotting. (G–I) Cell proliferation was detected by using MTT and EdU assays. (J, K) Cell apoptosis ratio was measured by using flow cytometry.