Research Paper Volume 14, Issue 3 pp 1321—1335

Figure 2. Effect of CTRP3 on OGD/R injury in H9C2 cells. H9C2 cells were transfected with pcDAN3.1-CTRP3 and CTRP3 siRNA or negative control (scramble and vector) to incubate for 24 h and then exposed to OGD/R injury. (A, B) Relative protein expression of CTRP3 was detected with Western blotting. (C–E) Effect of CTRP3 overexpression and inhibition on cell proliferation was assessed with CCK-8 and EdU assays. (F, G) Effect of CTRP3 overexpression and inhibition on cell apoptosis was detected with flow cytometry. (H–J) Effect of CTRP3 overexpression and inhibition on ROS, MDA and cTn-I production were determined.