Research Paper Volume 14, Issue 2 pp 678—707

Figure 5. KB1541 ameliorates senescence phenotypes. (A) Electron microscopy analysis revealed that DMSO-treated senescent fibroblasts exhibited less cristae wrinkle folds (blue arrow), whereas KB1541 treatment in senescent fibroblasts showed increased the number of cristae wrinkle folds (red arrow). Upper image: Scale bar 2 μm. Lower image: Scale bar 200 nm. (B) Comparison of cristae length between DMSO and KB1541-treated senescent fibroblasts. ^*P < 0.05, student t-test. Mean ± S.D., n = 6. (C and D) Flow cytometric analysis of ROS and mitochondrial mass using MitoSOX and MitoTracker green, respectively. ^**P < 0.01, student t-test. Mean ± S.D., n = 3. Flow cytometry data of ROS and mitochondrial mass were presented in Supplementary Figure 4A. (E) Flow cytometric analysis of autophagy level using Cyto–ID assay. ^**P < 0.01, student t-test. Mean ± S.D., n = 3. Flow cytometric data of autophagy level were presented in Supplementary Figure 4B. (F) Quantification of SA-β gal positive cells. ^**P < 0.01, student t-test. Means ± S.D., n = 4. Scale bar 100 μm.