PSMA3-AS1 induced by transcription factor PAX5 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-376a-3p to up-regulate LAMC1

Dongsheng Sun; Fujun Li; Lang Liu; Shaobo Yu; Haicun Wang; Xin Gao; Guanglin Liu; Yuqiao Zhao; Gongcai Qiu; Xingming Jiang

doi:doi:10.18632/aging.203828

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Research Paper Volume 14, Issue 1 pp 509—525

PSMA3-AS1 induced by transcription factor PAX5 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-376a-3p to up-regulate LAMC1

Figure 2. PSMA3-AS1 promotes CCA cell proliferation, invasion and EMT. (A) CCK-8 and (B) EdU assays determined the effect of PSMA3-AS1 on CCA proliferation. (C) Colony formation assay determined the effect of PSMA3-AS1 on colony-forming ability of CCA cells. (D) The effect of PSMA3-AS1 on CCA proliferation in vivo. (E) Knocking down PSMA3-AS1 restrained the tumor volume and (F) tumor weight in nude mice. (G) Wound healing and (H, I) transwell assays validated the effects of PSMA3-AS1 on migration and invasion of CCA cells. (J) The effect of PSMA3-AS1 on EMT process of CCA cells. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.