Research Paper Volume 14, Issue 1 pp 497—508

Figure 3. circCUL2 induces autophagy level in CRC cells via regulating miR-208a-3p. (A) The LC3 level was detected by immunofluorescence. n = 5. ^*P < 0.05, ^**P < 0.01 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (B) The protein level of LC3 in CRC cells. n = 5. ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (C) SW480 cells were treated with circCUL2 and Bafilomycin A1(Baf A1). LC3 and p62 puncta were measured by immunofluorescence analysis. (D) Immunoblotting analysis of protein levels of the SW480 cells treated for 100 nM Baf A1 for 24 h. n = 3, ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (E) The apoptosis level of SW480 cell after autophagy inhibitor (Baf A1, 100 nM, 24 h) and inducer (Rapamycin, Rap, 100 nM, 24 h). NC group, ^#P < 0.05 vs. circCUL2 group.