Research Paper Volume 14, Issue 1 pp 462—476

Figure 7. Activation of NLRP3 inflammasomes is critical for inducing apoptosis of neurons. (A) BV2 cells were treated with LPS without or with the indicated concentration of Bay11-7082 or JSH-23 for 24 h. IL-1β, TNF-α, and IL-6 protein expression were determined by western blotting. (B) N2a cells were treated with the IL-1β without or with the indicated concentration of Bay11-7082 or JSH-23 for 24 h. Bax and Bcl-2 protein expression were determined by western blotting analysis. GAPDH served as an internal control. The band densities were measured by Image J and protein quantities were estimated. The results represent the mean ± SD for the repeated experiments. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 vs. vehicle treatment. ^#P < 0.05; ^##P < 0.01 vs. LPS or IL-1β treatment.