Priority Research Paper Volume 13, Issue 24 pp 25607—25642

Figure 5. Serum from Dmp1-PPR^KO mice increases osteoclastogenesis and adipogenesis. (A) Representative TRAP staining images of BMMCs isolated from 3-month-old male control mice under osteoclastic differentiation in the presence of serum from 13-month old male control and KO mice. The total number (middle) and size distribution (right) of TRAP⁺ osteoclasts (OCs) per field were quantified. N = 13 per group. Data are presented as mean ± SEM. (B) CFU assay for osteoblasts was performed on BMSCs isolated from 13-month-old male control and Dmp1-PPR^KO animals. Data are presented as mean ± SEM. (C) Proliferation assay (left) and ALP activity assay (right) were performed on BMSCs isolated from 3-month-old male control mice under osteogenic differentiation in the presence of serum from male control and Dmp1-PPR^KO mice at 13 months of age. ALP activity assay was performed on day 6 of the osteogenic differentiation. N = 13–15 per group. (D) Representative Oil-red-O staining images of BMSCs isolated from 3-month-old male control mice under adipogenic differentiation in the presence of serum from male control and KO mice at 13 months of age. Quantification of lipid was performed by elution of Oil-red-O stain. N = 18 per group. Mann-Whitney test (A) or paired or unpaired t test (B–D) was performed. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. Data are presented as mean ± SEM.