Research Paper Volume 13, Issue 24 pp 26137—26147

Figure 5. Overexpression of TF abolished miR-545-induced downregulation of lipid oxidation and iron accumulation. (A) Transfection with OV-TF significantly increased TF expression in HT-29 and HCT-116 cells in the presence of OV-pre-miR-545. (B) A CCK-8 assay showed that overexpression of TF further augmented erastin and RSL3-induced reduction of cell survival in HT-29 and HCT-116 cells. (C) TF also decreased ROS content in HT-29 and HCT-116 cells treated with erastin and RSL3. (D) MDA levels were also reduced in HT-29 and HCT-116 cells transfected with OV-TF. (E) Fe²⁺ levels decreased when TF was overexpressed in HT-29 and HCT-116 cells. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. as indicated.