Research Paper Volume 14, Issue 1 pp 316—329

Figure 1. Effect of EpCAM on autophagy. (A) MCF-7 cells and MDA-MB-231 cells were treated with pCMV-SPORT 6-EpCAM plasmid, si-EpCAM sequence and M-EpCAM plasmid for 48 hr. Whole cell lysates were subjected to western blot to detect the expression of Beclin 1, p62 and the conversion from LC3-I to LC3-II. (B) MCF-7 cells and MDA-MB-231 cells were transfected with pCMV-SPORT 6-EpCAM plasmid or si-EpCAM sequence or M-EpCAM plasmid and pGFP-LC3 plasmid for 48 hr, images were collected. After transfection for 24 hr, the cells were observed under an inverted microscope. Arrow depicted the autophagosome. (C) Representative transmission electron microscopy images depicting ultrastructures of MCF-7 cells which were transfected with pCMV-SPORT 6-EpCAM plasmid, si-EpCAM sequence and M-EpCAM plasmid, respectively. (e–h) depicted boxed sections in panels (a–d) at a higher magnification, respectively. Arrows indicate autolysosomes.