Research Paper Volume 14, Issue 1 pp 297—315

Figure 6. TIMP-2 sustains the activation of ERK/MAPK in CRC cells. (A) Immunoblotting of phosphorylated ERK1/2 and ERK5 in DLD-1 5-FuS cells and DLD-1 5-FuR cells, HCT116 5-FuS cells and HCT116 5-FuR cells. (B) Immunoblotting of phosphorylated ERK1/2 and ERK5 in DLD-1 5-FuR cells and HCT116 5-FuR cells cultured with 5 μM of U0126 for 2 days, which down-regulates ERK/MAPK signaling. (C) Immunoblotting of phosphorylated ERK1/2 and ERK5 in DLD-1 5-FuS cells and HCT116 5-FuS cells cultured with 10 ng/mL of recombinant TIMP-2 for 6 h. (D) Immunoblotting of phosphorylated ERK1/2 and ERK5 in DLD-1 5-FuR cells and HCT116 5-FuR cells cultured with control IgG or 5 μg/mL of TIMP-2 neutralizing antibody for 6 h. Band intensities of western blotting for p-ERK5/ ERK5 and p-ERK1/2/ERK1/2 were analyzed. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by Student’s t-test.