Research Paper Volume 14, Issue 1 pp 297—315

Figure 4. TIMP-2 promotes CRC cell resistance to 5-Fu through an autocrine mechanism. (A) Changes in TIMP-2 protein levels in the cell culture medium (CM) of DLD-1 5-FuS cells and HCT116 5-FuS cells 3 days after treatment with 10 ng/mL of recombinant TIMP-2. (B, C) Relative cell viabilities of DLD-1 5-FuS cells and HCT116 5-FuS cells under increasing concentrations of 5-Fu for 3 days after culture with 10 ng/mL of recombinant TIMP-2. (D) Changes in TIMP-2 protein levels in the cell culture medium (CM) of DLD-1 5-FuR cells and HCT116 5-FuR cells for 3 days of culture with 5 μg/mL of TIMP-2 neutralizing antibody. (E, F) Relative cell viabilities of DLD-1 5-FuR cells and HCT116 5-FuR cells under increasing concentrations of 5-Fu for 3 days of culture with control IgG or 5 μg/mL of TIMP-2 neutralizing antibody. Data from triplicate wells for 3 independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by Student’s t-test or one-way ANOVA.