Research Paper Volume 13, Issue 24 pp 25886—25902
Structural, functional and molecular pathogenesis of pelvic organ prolapse in patient and Loxl1 deficient mice
Figure 6. Quantification analyses of type I collagen, type III collagen and elastin in vagina tissue of Loxl1 knockout mice. (A) qRT-PCR analyses showed mRNA expression level of Col1a1, Col3a1 and Elastin in vagina tissue (WT: wild type mice, KO: Loxl1 knockout mice, n = 3 in each group). (B) Western blots analyses of COL1A1 and COL3A1 in vagina tissue. Relative content of proteins were quantified on the right, the levels of protein expressed relative to TUBULIN, (n = 3 in each group). (C) Immunofluorescence staining for visualization (red) of COL1A1, COL3A1 and ELASTIN in vagina tissue (White dotted line indicated the interface between mucus layer and submucosa layer). The proportion of positive signal area (red) in each High Power Field of vision (HPF) of each protein were quantified on the right, (n = 6 in each group).