Research Paper Volume 13, Issue 24 pp 25778—25798
PKMYT1, exacerbating the progression of clear cell renal cell carcinoma, is implied as a biomarker for the diagnosis and prognosis
Figure 10. Downregulation of PKMYT1 inhibits epithelial-mesenchymal transition (EMT), promotes apoptosis, and decreases the G2/M ratio in the cell cycle. (A–B) Western blot analysis showing changes in EMT-related biomarkers in the ACHN and Caki-1 cell lines. Expression of E-cadherin was increased in the siRNA groups, while expression of N-cadherin, Vimentin, and Snail was decreased. (C) Representative images of flow cytometry results for the cell cycle in ACHN and Caki-1 cells. (D) Results showing reduction of G2/M ratio in cell cycle of ACHN and Caki-1 cells transfected with PKMYT1 siRNA versus a negative-control siRNA. (E–F) Western blot analysis of the protein expression levels of CCNB1 and CCND1 in ACHN and Caki-1 cell lines transfected with a negative-control siRNA or PKMYT1 siRNA. (G) Flow cytometry showing that inhibiting the expression of PKMYT1 contributed to a high apoptosis rate in ACHN and Caki-1 cells. *P < 0.05; **P < 0.01; ***P < 0.001.